gap43 recombinant protein  (Novus Biologicals)

Journal: Molecular Neurobiology

Article Title: Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury

doi: 10.1007/s12035-023-03702-3

Figure Lengend Snippet: Transplantation of lentivirus-mediated miR-28-5p-overexpressed BMSCs decreased the formation of glial scar and promoted axonal regeneration of SCI rats . A Co-immunofluorescent staining showed the glial scar (GFAP, red) and the axonal regeneration (GAP43, green) at 21 days after BMSC transplantation. B Quantification of GFAP immunoflurescent staining. C Quantification of GAP43 immunoflurescent staining. Data were presented with mean ± SD; # P < 0.05 vs. SCI group; & P < 0.05 vs. Lv-Con group

Article Snippet: Subsequently, tissues or cells were subjected to overnight incubation at 4 °C with primary antibodies, including microtubule-associated protein 2 (MAP2) at a dilution of 1:200 (Boster Biological Engineering Co.), neuron-specific enolase (NSE) at a dilution of 1:200 (Millipore), β3-tubulin at a dilution of 1:200 (Boster Biological Engineering Co.), neurofilament 200 (NF-200) at a dilution of 1:400 (CST), Notch1 at a dilution of 1:200 (Abcam), glial fibrillary acidic protein (GFAP) at a dilution of 1:600 (Boster Biological Engineering Co.), growth-associated protein 43 (GAP43) at a dilution of 1:200 (NOVUS), and myelin basic protein (MBP) at a dilution of 1:200 (Boster Biological Engineering Co.).

Techniques: Transplantation Assay, Staining